Since the inception of St. John Ambulance, Ghana (SJAG), it has operated an ambulance service as part of its emergency services conveying the colonial officers to hospitals in Accra during the colonial era. Under the Ghana Police Service, St. John continued to operate an ambulance service after independence.

Over the years of the agency's existence, SJAG had undertaken several duties for God and the country. SJAG has rendered unparalleled charitable services to humanity, provided emergency medical services to victims of disasters; provided first aid cover at national, religious and corporate gatherings, standby duties for health walks, cycling and other sporting events.

The contribution of SJAG to national development could be seen from the meritorious services it renders to institutions, corporate bodies and the public. Notable among other emergency medical services rendered was during the unfortunate May 9th stadium disaster in 2001 and the collapse of the Melcom supermarket at Achimota, a suburb of Accra, in November 2012.

SJAG deployed an emergency team that worked in collaboration with other Emergency Medical Service Organisations during the entire period of the rescue exercise.